1. Get the parts - (cutting files, bill of materials, links to suppliers here)
2. Z/Focus Frame: Assemble the frame for the Z Axis/Focus stacking unit
3. Connect Focus Knob: Connect the Focus stacking rig to the fine focus control on the microscope
4. XY Frame: Assembling the frame for the XY control
5. Electronics: Build or buy the Arduino based mother board.
6. Install Camera
7. Connect everything together
8. Software workflow.
Laser Cut Parts
-Download the laser cut parts and cut them or have them cut. Or go to Ponoko to our page on the project and order the parts you need. The complete list is:
1. Z-Focus stacking rig
2. Details on how to connect Focus stacking to your fine focus knob.
3. Front and middle XY plates.
4. parts for stepper motor 'sled' X 2 (x and y)
5. Stepper motor pulleys x 2 (slightly different sizes for X and Y knobs. And this will likely vary the most from microscope to microscope.
6. Allthread rod 'back' pieces. X 2.
3 Easy Stepper motor controllers
3 NEMA (17?) stepper motors, from spark fun ~300 milliamp draw.
various male and female headers
wire to connect 'everything' the way we want it.
(version two of the electronics should allow us to simplify this!)
Stepper motor crimps.
12 volt 1.25 or better amp wall wart - to power easy steppers.
USB cable to Arduino
USB cable to camera
Mechanical - Stock Drive Products
2 timing belt pullies for the XY steppers. The current version required me to drill out the pulley's, but there should be a matching size...
Two belts (size TK)
Four all thread rods, long enough to fit the width of the stage (dimensions tk). I used 4-40, but I think 6-32 might be a more durable choice.
-various metal and plastic nuts nad bolts to hold the stage together.
(camera page/link to come)Read about the Z-Axis page for more details. .
Building the Z Axis
(picture of parts) (description)
Read about the XY Page.
Building the XY Unit
If your arduino uses a USB to Serial adapter you may need to install the Virtual COM Port Drivers. See http://www.ftdichip.com/Drivers/VCP.htm